Coding

Part:BBa_K284000:Design

Designed by: Raíssa Estrela Curado, Taís Sineiro Herig and Gleidson Silva Teixeira   Group: iGEM09_UNICAMP-Brazil   (2009-08-16)

Lactate Permease from Kluyveromyces lactis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 547
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1273
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1461


Design Notes

Working yet.


Source

This part was amplified by PCR from Kluyverimyces lactis genomic DNA.

References

Queiros O, Pereira L, Paiva S, Moradas-Ferreira P and Casal M. 2007. Functional analysis of Kluyveromyces lactis carboxylic acids permeases: heterologous expression of KlJEN1 and KlJEN2 genes. Curr Genet 51, 161-169.